FastQCFastQC Report
Fri 31 May 2019
stdin:expA.r1-in.fastqc.1.html

Summary

[OK]Basic Statistics

MeasureValue
FilenameexpA.r1-in.fastqc.1.html
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1947567
Sequences flagged as poor quality0
Sequence length1-44
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTA203081.0427369122602714No Hit
GGCCTA155410.7979699799801496No Hit
CCCTTGTCCTATCCTTAAAATCCTTCTCGTTTCGCTCTTGCCCA66760.3427866666461282No Hit
AC60550.3109007289608008No Hit
GTCTAGGCTTGCTCGTCGTCACCCTTGTTCTCTTCCATCATCTC46830.24045385858355578No Hit
CTATTGTAGCAGGAGACGTCGATCCATGGGTCAACTTCTGTGTG37410.19208581784349396No Hit
C32340.1660533373177919No Hit
CG30400.1560921909233418No Hit
GTATATACATCGACGCAAGGTAGTCGCGTTTACGCTGGCAACCC29620.15208719391938763No Hit
GTGTATACGCTGCACGTTGGTTCACTTGACCATCTCGGCTTGTA29270.1502900798791518No Hit
GTCCTATCCTTAAAATCCTTCTCGTTTCGCTCTT25810.1325243239385346No Hit
ATATCACACCGTGTATACGCTGCACGTTGGTTCACTTGACCATC25520.1310352865909106No Hit
TACTTATGCAGTCTCGGATCCAAGTCG24590.1262600978554268No Hit
ACACATAGCCGTCCATTTTGGCCATCACTTACATCTTGCAATCT24510.12584932893194434No Hit
GTCG23950.12297394646756697No Hit
ATTCAGCACCGCTTTCGTCCTTTGATACTTGCTCTCAGACCCTT23510.12071471738841334No Hit
CTATAGTACATTCACAGCCGCGCAGGCTGAGGCAAGCGAAAGAC23180.11902029557904811No Hit
CCTTAAAATCCTTCTCGTTTCGCTCTTGCCCATCTCAAATTTTC22740.1167610664998945No Hit
CTTTGATACTTGCTCTCAGACCCTTGTCCTATCCTTAAAATCCT22570.11588818253749421No Hit
GACTATTGTAGCAGGAGACGTCGATCCATGGGTCAACTTCTGTG22540.11573414419118828No Hit
ATCATAAGCTGTTGCAAGAAGGTTCTTTATCGCCGTATGTGAGT21510.11044549430135138No Hit
CTTCACGACTGCTTCTTGTTCGCAATAGATTGATATTGAAATGG21230.10900780306916269No Hit
CTCAGACCCTTGTCCTATCCTTAAAATCCTTCTCGTTTCGCTCT21200.10885376472285677No Hit
CTTGTAAGGGCACGTTCGTGTTGCATCTGGACATACAGTAATTT20770.10664588175913844No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)