FastQCFastQC Report
Fri 31 May 2019
stdin:expA.r1-in.fastqc.2.html

Summary

[OK]Basic Statistics

MeasureValue
FilenameexpA.r1-in.fastqc.2.html
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1947567
Sequences flagged as poor quality0
Sequence length1-54
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGGCCTTCTCTCGAAATGTACGATGTCGCTAGGAGAAACAGTGAAGAGAGGA559622.873431311990807No Hit
CGATATGCCTCGTGATAACTCCACGTCTTGTTGTTCTTTTTTTGGCTT263561.3532782184130252No Hit
CGACTTGGATCCGAGACTGCATAAGTAAGACGCACAACATGCAAAGAC250041.2838582703444863No Hit
CGATATGGTCCTTGCTTGCAATCAATCGAT244221.253974831161136No Hit
TAGGCCGGTTGCGAGGGAGAACAAGAGAGAAGACAAAAGGGACCAGCCCGAGA213511.0962909106592995No Hit
TAGGC205271.0539817115406043No Hit
CGATCAGCTAGCATCGACACTCACATACGGCGATAAAGAACCTTCTTG190900.9801973436600642No Hit
TAGGCCGCTCGGTCAGCTGTGGTCAAGCTTAGAACGAGGATAGGTCAACCCTG190470.9779894606963457No Hit
TAGGCCATGGTGCTACAAGCCGAGATGGTCAAGTGAACCAACGTGCAGCGTAT188780.9693119671877785No Hit
TAGGCCGCCGTTGGTCCTGTGTTGGCAAGTAGTATGGGTCTTGGCTAAGGTGG177240.9100585499754309No Hit
TAGGCCATGTGGATGTTTGAATAGCGTCGACGGCGTCAGAAGAGGAGTCAAAT172430.8853610684510469No Hit
CGATCCAAAAAGCGGTTGCAGATAGCATCATCAGTAGTTCCAACTACA171680.8815101097933986No Hit
CGACCGCGATTCACCTTTTTCCGTGGTATCCTTCTCATATCTTCAAGT160770.8254914978534758No Hit
TAGGCCATAGGCACGAATATCGAAGAAAGAGCTGAGGTTTCAAAGAGAAGGCG157700.8097282404148356No Hit
TAGGCCTTTGCGAAAACACCGCTTGAACAAGTGATACCGAATACGACGTGGTC157610.8092661253759178No Hit
TAGGCC156990.8060826662189287No Hit
CGAACAAACATCGTACGTCGATTGACGCTAACTTGTTTCAGCCGTAGA149510.7676757718733168No Hit
CGAAGCAACTCAACGTGGTCCATTTCAATATCAATCTATTGCGAACAA148500.7624898142143505No Hit
TAGGCCCTAACATGCAGAAAGCTGAAGCTCAATGTGACCGGAACAAGAACAGC146440.7519125144346767No Hit
CGACCGCTTCCCCCCTCCCGTCGCTCGTCCAGCCTTCTTGGCATTCGC144990.7444673276965568No Hit
CGATCATCTCCTTCGCGATCCACTTTTCTCGAAAATTCACGATTTTCG141290.7254692649854921No Hit
TAGGCCGTCGAAGTCGTATGAAGGAATCAAGAGCTGATGTTTGTGTCTTTGCG124490.6392077910541717No Hit
TAGGCCTGTAAGCTTTTCTTGGCTAAGCCTATAAAAGTGCGAGACAAGTCTTT122210.627500876734921No Hit
TAGGCCATTGACTGAACAAGGCAAAGGAGCGAGACGGTGGTAGAGAAGGTAGG120940.6209799200746366No Hit
CGATTTTGGGTGGCATTTCCCAGCAGTCGGCGGCAGCTCATCCGCATT120820.620363766689413No Hit
CGATCCATGCCTCCCTGTTTTGTGCATCTCTTGTGTCGGCGGTCGTGA119260.6123537726815047No Hit
TAGGCCGGTGTTTAGGCCGATGTGGTCTTGATAGAATTGGTGTATTTGAGTGG118970.6108647353338807No Hit
TAGGCCGTGTTTTGACGGATGATCGAAAGACTCGAGATTGCAAGATGTAAGTG117930.6055247393286085No Hit
CGATTTGGGCGGCCCTTTCTTCATGTTGAAATCGACACCATTCGTGAA113550.5830351407679427No Hit
CGACTCGTCCGCAAAAGATCCGCCCAGCCGCCCAGTCGCCCTTTTACC112330.576770914684835No Hit
TAGGCCCTTAGCGCACAAGGAAAAAGTATCAGGGAAGGGTCTAAGTGAGATGA110120.5654234231736315No Hit
CGACTTTCGAGCTCGACAAACCACTTTGTCTTTCCCAGCTGAGACATA109920.5643965008649253No Hit
TAGGCCGTCAGAGTTTTCCGAAGCAAATAGTTGAGTCAACGTGATTCTTGTTA103730.5326132554104686No Hit
TAGGCCGCACTTGCGTGACAATCAGCGTCGTTGTCCTTCGGAAGAATAGGTCG102990.5288136428682556No Hit
CGACAGAACAAACACACACATCTTTGATGTCATTCTACTTGCTCTTGC100800.5175688435879228No Hit
TAGGCCGTATCGTTGAGGGCTGTACAAGTTCGAGTAAGGAGAACGTCAGCCGA100110.5140259616228864No Hit
TAGGCCGCGGCGTCAGTGATGTCAGGTGCAGTTGTCGATAAGCAAAGAAGGAC97420.5002138565707881No Hit
TAGGCCTTGAGCGCAAGATCGAGAAGCTGGGAATTCCGGTGGAGCGAATCAAG97300.49959770318556435No Hit
CGAAGGACAGAAATGGTTGGATATAGAGCACAGTGCCGTGAGTAGTCG97050.49831405029968157No Hit
TAGGCCTCGGGAAGGTGCTGATGGTGTTGCAGAGGCAAGCGCTTCGTCTTGCT96700.4965169362594457No Hit
TAGGCCTTGAACAGGCTTCCGAGCGTGGTATGTAGCGAGACAAGGGATGGTTT95220.4889177111750199No Hit
CGACCCTGACGAGCAGAATGGTCGATCTTCACATTCCTGTCTAACCAC95160.488609634482408No Hit
TAGGCCACTTCTGTTATTGTAGCAGCAATAGCTTGAAGCGAATGGACAAGTAG94320.484296560785842No Hit
CGAGCTGCACAGCGGATGGCAAAGTGAAAGTGAACGGTGTTGGCGGCC93900.48214002393755906No Hit
CGACTATGTCTCTCGTCTCCTCACACGAATTCGTTCCGTGAATGAATC93180.4784431036262167No Hit
CGAATTCATCTCTGCAGCTCGCCCGAGAGAGTTGCGGTCGGATAGCCC91770.471203301349838No Hit
CGACTTAAATACAGTCATACCTTTGCCTTTTTCTAATCACCACAAGTT90000.46211503891778816No Hit
CGACGTCATTTCTGTGATGCACTTGTCTCATTTCTGGAATACAAGAGG89070.45733985018230433No Hit
CGACATCGGCCCCGCACACTAATCACCATCAGGAATATACCCAACCCA87030.44686524263350114No Hit
TAGGCCTTGTACGCTCTTGAGCAAGCCCGCAATTGCAGAAGCGTTAACCGTAG85550.43926601754907535No Hit
TAGGCCGCTTGTGTAGATGTGCGACGGCGCTGCGTCGAACCAAGATCAAGTGC81430.41811141798972773No Hit
CGAAGCTGAGACCTCAAATCGAATCGTCTGAACACGGAAAGCACGAAC80640.4140550748703382No Hit
TAGGCCGCTGAGTGCAAGGGGCGACAGAAAACACTAGAAAATGACAGAAGTGG79800.4097420011737722No Hit
CGATCCTTCCGTACTGCATTGCATATCTCGCTCCGCTTAAATTATATA78390.4025021988973935No Hit
TAGGCCGTGTCATATGCCAGTGAAAGGGTTGGCTATTCAAGGGAGACAGATGT77910.40003758535649864No Hit
CGACCCGCGTACCACCCGTCACACTTGTCTCGCACTTCTCAAGCTTGG77730.3991133552786631No Hit
TAGGCCGCGAAATAGGAAAAATGAGGTGAGAGACGAGAGGCTTGGAACGACAC77430.3975729718156038No Hit
CGAACCTCGTCATTCCGATCCTAGACAAACGAGAACGCTCAGATGGGC74610.38309336726284643No Hit
GACCACGCGAGCTGAGGCCTACTGTATGTGATGGTTTCAATCCAACTTGTTCA73390.3768291411797386No Hit
TAGGCCGGCGATGCGCCTTGTTTGCAATGAACTGTAAAACGATCAGTAGGACT72410.37179722186707825No Hit
CGACCGCCCACATTGTAGATGTATATTCCTAGCGAATTGCCGAAATCA72300.37123241459728984No Hit
CGAGGCTTCTCACTTCTGTGAGAGAAGTGACGATTGCTAAGCTAGTGA71220.36568703413027637No Hit
CGAGCATACGTAGGACCATATCAATCTTTGAACTAATACCACTGACCT70200.3604497303558748No Hit
CGACGCTTCATTTGAAAACGTGAAGTTGCTCCCCATCCCTTCTCTTTC69650.3576256940069327No Hit
CGATCAGGGATGTCGACGCATGCGTTGGCAGAGCAAATGGGCTTTACC69010.3543395426190729No Hit
TAGGCCGCCTGAATCCAAAAACGGCACGGTGATGAGTCAAGAAGCAGGAGAAA68550.3519776213090487No Hit
CGACTGTTGGGGTGACCAGGCTGAAAACTCGGCACAAGCTGCACTTGA68150.3499237766916363No Hit
CCCCTTTTCCCCCTCTTCCACCACAGAGCCTCATCACCCGAATTCTGCCGCCC67350.3458160874568115No Hit
CGACCATTTTCACACACTTGACGCAGCCTCAACAAATGCAAAACTAGC64430.3308230217497011No Hit
TAGGCCGTGTTTTTAGAACAAGTCAGCACGAAAAATCCCTATCAAGAGTGAGG63990.3285637926705474No Hit
CGACTAAAACTGGTGCGGCTTTGCCATCGTTCCACAAGTTCTAGTCTC63720.3271774475537941No Hit
TAGGCCGCTGGCAAGGGGTTTGCTTAAATGACCTGGTGAGCTTTCTGTTTGTT63330.32517494905181693No Hit
CGAGTCCGAAGCTATTACGATCAAGATGCGCTGGCTACCAACCCACTT62950.3232237966652752No Hit
GT60070.30843611541990595No Hit
CGACGCTAAGTGCTCTTGTACTAACTTGAATGCACATTCCACATTCTT59460.3053040023783521No Hit
TAGGCCTTGATGAAAAGAGAGAGGTGCTATGAAGGGGGATTCGTTGGCGTGAG58930.30258265826028063No Hit
CGACAGTGTGAGTGCACACAACCCCAATATAAGGACATTTGCCTTTAT57580.2956509326765138No Hit
CGACTGCACGAAATGGTTTTTCAGGTAGCTGTAAACATCAATCGTGAA56470.28995151386319445No Hit
TAGGCCGTTGGGGGAAGTAGAGAGACAAAAGAGGTTCTGGGTTGACAAGGATG56310.2891299760162295No Hit
TAGGCCGCTTGATCTGGAGGAAGAGAATGGAATACTAAGGTGAAACGAGCAAG55680.285895170743805No Hit
TAGGCCCTCAACAACTGTGAATGGTCGCGGCCAAGCTATATTCCTCACTGATA55000.2824036348942039No Hit
TAGGCCGATGCTGAGCTGGTTGTGTGTTGGTCATACAATCCTTGAGATGAACG54450.2795795985452619No Hit
TAGGCCTTGCGTTTCTACCCTGTGGAATAACTCACGACTGTTTCGGGACAAAA54350.27906613739090874No Hit
CGAGAGCTTTTTCAACACTTCGTCTTGCGCTGAGCTCTCGGCTACTGT53210.2732126802312834No Hit
TAGGCCGCGATAAGCCCAGCTTGAACCGATCTGATCAATACCACTTTTGGATC52990.27208306569170665No Hit
CGAGACTGCTTGTATTAGAAACGCTGCTTCTACTTCCGCTTTACTTTT52280.26843749149579965No Hit
TAGGCCATGACGATAGTTTGACGGCCGCTAAAGGAAGTCGTAGATGTTTTTGC52110.2675646075333994No Hit
TAGGCCGCCCGTGTAGGTTTGCAGGAAAAAAGCGTGTATGTGGGTGTTTGGTG51710.26551076291598696No Hit
TAGGCCTTAAAGCGAATCGAATGGAATGCTCCCCAGCAGAGAGATCCAAAGAA51410.2639703794529277No Hit
CGAGCGTCTTGCCCTCGCGTCGAGCGAGAGAGCGGTGCTGGATCAGCT51060.2621732654126918No Hit
CGACGAGTCCATATGGGCTCTGCGTTCTCTTGGACTTCTGCCAAGCTC49920.2563198082530665No Hit
CGATATGGTCCTTGCTTGCAATCAATCGATGATTCGTTTTTACAGATC49260.25293096463433606No Hit
CGAAAATCGTGAAAGACAAAGGTGGAGCAAGTGCTGGTTGTTATTATT46710.23983770519833206No Hit
CGATGCTGACACGTGCAGGAAAAGGGAAGACTAGCGTTGCTGCGTAGG46050.23644886157960163No Hit
TAGGCCTTCTCTCGAAATGTACGATGTCGCTAGG45480.233522132999789No Hit
CGAGTTAGCACGCCACGAGGCTGGCAAAGACGTTCCCGAGGACATTGT42950.2205315657946556No Hit
CGACTCCGCTCCTAGATTCGTTTTGTGCACACGTCAAATCAATCAAAT42840.2199667585248672No Hit
TAGGCCGTGCTAGTATGTAAACGGATAATGTGAGCGATTGGCGTTGTACTGGA42800.21976137406312596No Hit
TAGGCCGTATGGATGACGCAAGGTTTTGATGGGGCCACAATATGTGTCGTGAG41840.2148321469813362No Hit
CGACCAGGTCAGCTCGGCTTGCCGCCACAGCACAACCTGGCTTGTAGC41770.21447272417328903No Hit
CGACCATTTCTAGCGAGTCCTTGCAGTGGAGCATGGCGAACAATCGTG41720.2142159935961125No Hit
CGACAATTGCTCGTTTCGCACTCGCTCACGCCTTGAACGTTGCTCTTG41150.21128926501629985No Hit
TAGGCCGACGCCCCCAGCACATGTGCGTCCAGCGTCGGTTCCATCCCGTTTAG40370.20728426801234567No Hit
TAGGCCGTTACTGGATAGCGATAAAGGCAAGGCAAGAGAGATGTGGTCTGAAG40320.2070275374351691No Hit
TAGGCCTTGTCCAGGGAACGGGTTCAAAAGTGGTAGAGAATGTAGGCTTTGAG37960.194909854192436No Hit
TAGGCCCTGGGTCTCGCTGTGGCTGTGGCTGTAGTTTCGGAAATCATGGCCCT37520.19265062511328238No Hit
TAGGCCTTCTCTTGGCACGTGTTTTGGGAGTGACGATGTCGATGTGGAAGTAA35880.18422986218189155No Hit
TAGGCCTTTCTTACTACTTGTATACAGAAGATCGTGAAACGCCTCCAAATGCT35460.18207332533360857No Hit
TAGGCCGTTCGGTATTCGTGCAGCAGTGATTGCTCCCGCACCGGCTTTTATGG34150.17534698421158296No Hit
TAGGCCTCGTGTCTTTTAGTACAACCGATGCGACAGGAGTCCAGGAAGAGGTA34010.17462813859548862No Hit
CGAAGTAGCAGTCACGAGTGGGATTTTGGCGCAGATGTGAGCGTTTCA33890.17401198521026492No Hit
G32000.16430756939299138No Hit
TAGGCCTTGATCTTGACTTCAGTAGTGAGGCAGTAATGCTTGAAGGCTTGAAG31660.16256180146819083No Hit
TAGGCCTGTAAGCTTTTCTTGGCTAAGGATGGCGTCAGACAAGGCGCGCCCTG30640.15732449769378923No Hit
CG30490.15655430596225955No Hit
CGATGCTTTTCCATACCCCTATGCCGCGTAGGTCCTTGTTTTCCTCGC30420.1561948831542124No Hit
CGAGCGCCTCCAATTACGGACGGTGCGCAAGCTCCTCGTAGATGGGTT28320.14541219891279736No Hit
CGAGGCTCAATAGCGACTAATAAAGTCAAGAAGGTGGCGCCATATCGT28000.14376912321886745No Hit
CGACTTGGATCCGAGACTGCATAAGTA25740.13216490113048743No Hit
CGATGGCTCACTGGTGTAGAAGTTGTTGTCGTGAGGCAGCTAGATGCA24740.12703028958695645No Hit
CGAC24170.1241035610071438No Hit
CGAAGTACACGGTAGTCAAGGCTGTTCGTTAAATGTCTTTTTCAAGTA23030.11825010384751847No Hit
TAGGCCTCGTGCGATTCGGTAAGGGAGGCGGGTTAGCGAGGAGTGGAATGTTG22340.1147072218824821No Hit
CGACTATGGGCGCACGATGCTACCTCTGCTTTCTTGTCTACCGTGATG21930.11260203114963438No Hit
TAGGCCGCTCGGTCAGCTGTGGTCAAGCTTAGAA21920.11255068503419909No Hit
TAGGCCTTGTAGTTATGAGAGAATTCAAAGTGGTTGATGGGATGGAGAGAGTT21800.11193453164897535No Hit
CGATTCTACACACCCACGACCCATGTCTGGTATACAAATTGCAAATGT21070.10818626522219775No Hit
CGAGCGCCCCCCACACTTTCATTCAATTCCATATCTTGCTTGATTGCT21030.1079808807604565No Hit
TAGGCCTTGGACGCTCCTCTCGTTTCGTCGAACCAATATGCTTGACATCGACG20780.10669722787457377No Hit
TAGGCCGGTCGCGGACACACCTGCTTTGGGTGCCAGTGGAGGTCCAAGTCGGT20120.10330838425584331No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)