FastQCFastQC Report
Fri 31 May 2019
stdin:expA.r1-out.fastqc.1.html

Summary

[OK]Basic Statistics

MeasureValue
FilenameexpA.r1-out.fastqc.1.html
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2157059
Sequences flagged as poor quality0
Sequence length1-44
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTA272991.265565754112428No Hit
GGCCTA234161.0855521337153968No Hit
AC77360.35863645825172147No Hit
GTCTAGGCTTGCTCGTCGTCACCCTTGTTCTCTTCCATCATCTC75920.3519607020484836No Hit
GGCCTACTCGGCTCGTGATTCAGCACCGCTTTCGTCCTTTGATA71240.3302644943879606No Hit
CCCTTGTCCTATCCTTAAAATCCTTCTCGTTTCGCTCTTGCCCA60100.27862010264902354No Hit
TGTCTACTCTGTGTGCCACGTTCCCTCACCGTGATCCACTTCAA48160.22326695746384315No Hit
GTGTATACGCTGCACGTTGGTTCACTTGACCATCTCGGCTTGTA47940.22224705026612623No Hit
CG36180.1677283746063506No Hit
C35250.16341694872509283No Hit
GTCATGTGAATGGCGCGGGACTCCTCTGTCAGCCCTCGCACATT34730.1610062589850347No Hit
GACGTACACCGTGCAGCAGCAGCTCTCAGTCACATCGCAGCGAC34330.1591518822619131No Hit
CTATTGTAGCAGGAGACGTCGATCCATGGGTCAACTTCTGTGTG32060.1486282943581979No Hit
CGCCAACACCGTTCACTTTCACTTTGCCATCCGCTGTGCAGCTC31830.14756202774240296No Hit
GCATACACGCCTTGGCTGAATAATCACGACCGCCGACACAAGAG30050.13931005132451177No Hit
ACACATAGCCGTCCATTTTGGCCATCACTTACATCTTGCAATCT29100.13490590660709792No Hit
CTTCACGACTGCTTCTTGTTCGCAATAGATTGATATTGAAATGG29010.13448867184439553No Hit
TCCATACACTACGCTACAACTGCGCACCAGTTTACTGCTGCACT26040.1207199246752175No Hit
GACCTGGCCTACTCGGCTCGTGATTCAGCACCGCTTTCGTCCTT25980.12044176816674926No Hit
ACCCTAGACACACCAGGGTTCAATAGAGAAAGCTGGTCGCGTGT25960.12034904933059319No Hit
GCCTAGAGGTCGTGCACTTGAATCGTCCTTGATC25780.11951457980518844No Hit
CACATATATCACACCGTGTATACGCTGCACGTTGGTTCACTTGA25660.11895826678825197No Hit
CTGCTGCGCTGAGCAAACACGAAAGAAACCGCTTGATCTTGGAC25440.11793835959053507No Hit
CTTGTAAGGGCACGTTCGTGTTGCATCTGGACATACAGTAATTT24730.1146468409069942No Hit
TACTTATGCAGTCTCGGATCCAAGTCG24490.11353421487312124No Hit
CTGCTACACAGTGCGCCCATCCACTTGCCCCGTTCCAACAAGTT24310.11269974534771648No Hit
GTCG23240.10773928761336617No Hit
GGTATGTGCTACGTCAAACACATGGTATGTCTCAGCTGGGAAAG22730.1053749572913861No Hit
ATGTACAGATGATCGGGTTGAAAGCCAAAAAAAGAACAACAAGA22600.10477228485637156No Hit
GAGTAGGCGTGGCTTCTGTTGTGCGCACTGCTCCAATGTTGGAC22260.10319606464171818No Hit
ATCATAAGCTGTTGCAAGAAGGTTCTTTATCGCCGTATGTGAGT21700.10059993722934793No Hit
GACTATTGTAGCAGGAGACGTCGATCCATGGGTCAACTTCTGTG21630.10027542130280165No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)