FastQCFastQC Report
Fri 31 May 2019
stdin:expA.r1-out.fastqc.2.html

Summary

[OK]Basic Statistics

MeasureValue
FilenameexpA.r1-out.fastqc.2.html
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2157059
Sequences flagged as poor quality0
Sequence length1-54
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGGCCTTCTCTCGAAATGTACGATGTCGCTAGGAGAAACAGTGAAGAGAGGAT527162.4438830834019836No Hit
TAGGCCATGGTGCTACAAGCCGAGATGGTCAAGTGAACCAACGTGCAGCGTATA306141.4192472250411325No Hit
CGATATGGTCCTTGCTTGCAATCAATCGATGATTCGTTTTTACAGATCC299341.387722820748065No Hit
CGATATGCCTCGTGATAACTCCACGTCTTGTTGTTCTTTTTTTGGCTTT279371.295143062846218No Hit
TAGGCCATGTGGATGTTTGAATAGCGTCGACGGCGTCAGAAGAGGAGTCAAATG277361.2858248198125317No Hit
TAGGC272531.263433220880838No Hit
TAGGCC234691.0880091828735328No Hit
CGACTTGGATCCGAGACTGCATAAGTAAGACGCACAACATGCAAAGACC229031.061769752241362No Hit
TAGGCCGGTTGCGAGGGAGAACAAGAGAGAAGACAAAAGGGACCAGCCCGAGAT226141.0483718804168083No Hit
CGACCGCGATTCACCTTTTTCCGTGGTATCCTTCTCATATCTTCAAGTG223491.0360866346261275No Hit
TAGGCCGCTCGGTCAGCTGTGGTCAAGCTTAGAACGAGGATAGGTCAACCCTGC222831.033026913032977No Hit
TAGGCCGTCGAAGTCGTATGAAGGAATCAAGAGCTGATGTTTGTGTCTTTGCGG214440.9941313612655008No Hit
CGAAGCAACTCAACGTGGTCCATTTCAATATCAATCTATTGCGAACAAG212810.9865747761187803No Hit
CGATCCATGCCTCCCTGTTTTGTGCATCTCTTGTGTCGGCGGTCGTGAT192920.8943658935615576No Hit
CGACTTTCGAGCTCGACAAACCACTTTGTCTTTCCCAGCTGAGACATAC178740.8286282387268963No Hit
CGATCCAAAAAGCGGTTGCAGATAGCATCATCAGTAGTTCCAACTACAG172220.7984018981400137No Hit
CGACAGAACAAACACACACATCTTTGATGTCATTCTACTTGCTCTTGCA167500.7765202528071786No Hit
TAGGCCGCACTTGCGTGACAATCAGCGTCGTTGTCCTTCGGAAGAATAGGTCGT157620.7307171477460747No Hit
TAGGCCGGTGTTTAGGCCGATGTGGTCTTGATAGAATTGGTGTATTTGAGTGGC156950.7276110667348459No Hit
CGAGCTGCACAGCGGATGGCAAAGTGAAAGTGAACGGTGTTGGCGGCCG155850.7225115307462614No Hit
TAGGCCGCCGTTGGTCCTGTGTTGGCAAGTAGTATGGGTCTTGGCTAAGGTGGA149590.693490535029408No Hit
TAGGCCGCGAAATAGGAAAAATGAGGTGAGAGACGAGAGGCTTGGAACGACACC142650.661317098883248No Hit
CGAAGGACAGAAATGGTTGGATATAGAGCACAGTGCCGTGAGTAGTCGC135890.6299781322624926No Hit
CGAACAAACATCGTACGTCGATTGACGCTAACTTGTTTCAGCCGTAGAC131080.6076792521669551No Hit
TAGGCCGCTTGTGTAGATGTGCGACGGCGCTGCGTCGAACCAAGATCAAGTGCA127790.5924270036192798No Hit
CGACGCTTCATTTGAAAACGTGAAGTTGCTCCCCATCCCTTCTCTTTCG127710.5920561282746555No Hit
CGACTGTTGGGGTGACCAGGCTGAAAACTCGGCACAAGCTGCACTTGAA123880.574300471150766No Hit
CGATCAGCTAGCATCGACACTCACATACGGCGATAAAGAACCTTCTTGC117910.5466238985581757No Hit
CGACATCGGCCCCGCACACTAATCACCATCAGGAATATACCCAACCCAT117660.5454649131062248No Hit
CGACTTAAATACAGTCATACCTTTGCCTTTTTCTAATCACCACAAGTTC116060.5380474062137383No Hit
CGATTTGGGCGGCCCTTTCTTCATGTTGAAATCGACACCATTCGTGAAT114300.5298881486320031No Hit
TAGGCCACTTCTGTTATTGTAGCAGCAATAGCTTGAAGCGAATGGACAAGTAGT113510.5262257546038379No Hit
TAGGCCGCGGCGTCAGTGATGTCAGGTGCAGTTGTCGATAAGCAAAGAAGGACA113190.5247422532253406No Hit
TAGGCCTGTAAGCTTTTCTTGGCTAAGCCTATAAAAGTGCGAGACAAGTCTTTC112550.5217752504683459No Hit
TAGGCCGTGTTTTGACGGATGATCGAAAGACTCGAGATTGCAAGATGTAAGTGA112140.5198745143271464No Hit
TAGGCCTTGAACAGGCTTCCGAGCGTGGTATGTAGCGAGACAAGGGATGGTTTG109990.5099072394403676No Hit
CGACGTCATTTCTGTGATGCACTTGTCTCATTTCTGGAATACAAGAGGT109210.5062912048302805No Hit
TAGGCCGTATCGTTGAGGGCTGTACAAGTTCGAGTAAGGAGAACGTCAGCCGAA106490.49368144311305345No Hit
TAGGCCATTGACTGAACAAGGCAAAGGAGCGAGACGGTGGTAGAGAAGGTAGGA102150.4735614556671839No Hit
CGATTTTGGGTGGCATTTCCCAGCAGTCGGCGGCAGCTCATCCGCATTT102120.47342237741294974No Hit
TAGGCCCTTAGCGCACAAGGAAAAAGTATCAGGGAAGGGTCTAAGTGAGATGAA101720.4715680006898281No Hit
GACCACGCGAGCTGAGGCCTACTGTATGTGATGGTTTCAATCCAACTTGTTCAA99170.45974634907992784No Hit
TAGGCCATAGGCACGAATATCGAAGAAAGAGCTGAGGTTTCAAAGAGAAGGCGG93550.43369235612006907No Hit
CGATCAGGGATGTCGACGCATGCGTTGGCAGAGCAAATGGGCTTTACCG92310.427943788278392No Hit
CGACTATGTCTCTCGTCTCCTCACACGAATTCGTTCCGTGAATGAATCA91720.4252085826117876No Hit
TAGGCCTCGGGAAGGTGCTGATGGTGTTGCAGAGGCAAGCGCTTCGTCTTGCTC88680.4111153195160633No Hit
CGAGCGTCTTGCCCTCGCGTCGAGCGAGAGAGCGGTGCTGGATCAGCTG88440.4100026934821904No Hit
CGAGCATACGTAGGACCATATCAATCTTTGAACTAATACCACTGACCTC86910.40290970251625013No Hit
CGACCCGCGTACCACCCGTCACACTTGTCTCGCACTTCTCAAGCTTGGT86430.4006844504485042No Hit
CGACCGCCCACATTGTAGATGTATATTCCTAGCGAATTGCCGAAATCAT84810.3931742247198616No Hit
TAGGCCTTGAGCGCAAGATCGAGAAGCTGGGAATTCCGGTGGAGCGAATCAAGA83040.38496860772004843No Hit
TAGGCCGTGTCATATGCCAGTGAAAGGGTTGGCTATTCAAGGGAGACAGATGTG82290.38149165136419544No Hit
CGACCCTGACGAGCAGAATGGTCGATCTTCACATTCCTGTCTAACCACA80350.3724979242570555No Hit
TAGGCCGGCGATGCGCCTTGTTTGCAATGAACTGTAAAACGATCAGTAGGACTT79350.3678619824492515No Hit
TAGGCCTTGTACGCTCTTGAGCAAGCCCGCAATTGCAGAAGCGTTAACCGTAGA78480.363828713076462No Hit
GT77150.3576629104720826No Hit
TAGGCCGCGATAAGCCCAGCTTGAACCGATCTGATCAATACCACTTTTGGATCG76730.3557158149128049No Hit
CGAATTCATCTCTGCAGCTCGCCCGAGAGAGTTGCGGTCGGATAGCCCG70520.32692661628634173No Hit
TAGGCCGTTGGGGGAAGTAGAGAGACAAAAGAGGTTCTGGGTTGACAAGGATGT69180.3207144542638843No Hit
TAGGCCGTGTTTTTAGAACAAGTCAGCACGAAAAATCCCTATCAAGAGTGAGGC68460.3173765761622654No Hit
TAGGCCGATGCTGAGCTGGTTGTGTGTTGGTCATACAATCCTTGAGATGAACGC67890.31473408933181707No Hit
CGAGGCTTCTCACTTCTGTGAGAGAAGTGACGATTGCTAAGCTAGTGAT66900.31014450694209106No Hit
CGATGCTGACACGTGCAGGAAAAGGGAAGACTAGCGTTGCTGCGTAGGA66690.30917095916245224No Hit
TAGGCCGTCAGAGTTTTCCGAAGCAAATAGTTGAGTCAACGTGATTCTTGTTAC65960.3057867216427553No Hit
TAGGCCTTGATGAAAAGAGAGAGGTGCTATGAAGGGGGATTCGTTGGCGTGAGG64570.29934276252990766No Hit
TAGGCCTTAAAGCGAATCGAATGGAATGCTCCCCAGCAGAGAGATCCAAAGAAC63180.29289880341706No Hit
TAGGCCGTGCTAGTATGTAAACGGATAATGTGAGCGATTGGCGTTGTACTGGAT62590.29016359775045564No Hit
TAGGCCCTAACATGCAGAAAGCTGAAGCTCAATGTGACCGGAACAAGAACAGCC62410.28932912822505086No Hit
CGACTGCACGAAATGGTTTTTCAGGTAGCTGTAAACATCAATCGTGAAT60850.2820970590048765No Hit
CGACTCCGCTCCTAGATTCGTTTTGTGCACACGTCAAATCAATCAAATT60450.2802426822817549No Hit
TAGGCCGCTGAGTGCAAGGGGCGACAGAAAACACTAGAAAATGACAGAAGTGGC58850.2728251753892684No Hit
CGACAGTGTGAGTGCACACAACCCCAATATAAGGACATTTGCCTTTATC57970.2687455465984009No Hit
TAGGCCTTGTCCAGGGAACGGGTTCAAAAGTGGTAGAGAATGTAGGCTTTGAGG57370.2659639815137184No Hit
CGACCATTTTCACACACTTGACGCAGCCTCAACAAATGCAAAACTAGCA56010.25965910065510495No Hit
CGAGTCCGAAGCTATTACGATCAAGATGCGCTGGCTACCAACCCACTTT54360.2520097966722282No Hit
TAGGCCGTATGGATGACGCAAGGTTTTGATGGGGCCACAATATGTGTCGTGAGC53650.2487182779886874No Hit
TAGGCCGTTACTGGATAGCGATAAAGGCAAGGCAAGAGAGATGTGGTCTGAAGG53210.2466784635932536No Hit
CGACCATTTCTAGCGAGTCCTTGCAGTGGAGCATGGCGAACAATCGTGA52440.24310878840124445No Hit
CGAAGCTGAGACCTCAAATCGAATCGTCTGAACACGGAAAGCACGAACC52030.24120805226004483No Hit
CGATCCTTCCGTACTGCATTGCATATCTCGCTCCGCTTAAATTATATAC51420.23838012775728432No Hit
CGACTCGTCCGCAAAAGATCCGCCCAGCCGCCCAGTCGCCCTTTTACCT50620.23467137431104113No Hit
TAGGCCTTCTCTCGAAATGTACGATGTCGCTAGG47240.2190018910006634No Hit
TAGGCCTTTGCGAAAACACCGCTTGAACAAGTGATACCGAATACGACGTGGTCA44750.20745839589923132No Hit
TAGGCCATGACGATAGTTTGACGGCCGCTAAAGGAAGTCGTAGATGTTTTTGCC44240.20509406557725124No Hit
CGAACCTCGTCATTCCGATCCTAGACAAACGAGAACGCTCAGATGGGCG44160.20472319023262692No Hit
CGACTAAAACTGGTGCGGCTTTGCCATCGTTCCACAAGTTCTAGTCTCA43290.20068992085983742No Hit
CGACGAGTCCATATGGGCTCTGCGTTCTCTTGGACTTCTGCCAAGCTCC42040.19489499360008233No Hit
CGATCATCTCCTTCGCGATCCACTTTTCTCGAAAATTCACGATTTTCGC41900.19424596174698977No Hit
CGAGGCTCAATAGCGACTAATAAAGTCAAGAAGGTGGCGCCATATCGTT41360.1917425531707756No Hit
TAGGCCTTGCGTTTCTACCCTGTGGAATAACTCACGACTGTTTCGGGACAAAAG40980.18998089528381004No Hit
CGAGAGCTTTTTCAACACTTCGTCTTGCGCTGAGCTCTCGGCTACTGTT40930.18974909819341984No Hit
CGACAATTGCTCGTTTCGCACTCGCTCACGCCTTGAACGTTGCTCTTGG40070.18576218823870835No Hit
TAGGCCGTCGAAGTCGTATGAAGGAATCAAGAGC39930.1851131563856158No Hit
TAGGCCCTCAACAACTGTGAATGGTCGCGGCCAAGCTATAT38440.17820560309198774No Hit
CGATTCTACACACCCACGACCCATGTCTGGTATACAAATTGCAAATGTG38430.17815924367390973No Hit
CGAAAATCGTGAAAGACAAAGGTGGAGCAAGTGCTGGTTGTTATTATTC38310.17760293065697322No Hit
TAGGCCGCCCGTGTAGGTTTGCAGGAAAAAAGCGTGTATGTGGGTGTTTGGTGC37360.17319878593955937No Hit
CGAGACTGCTTGTATTAGAAACGCTGCTTCTACTTCCGCTTTACTTTTG36500.16921187598484788No Hit
CG36230.1679601716967408No Hit
CGAGCGCCTCCAATTACGGACGGTGCGCAAGCTCCTCGTAGATGGGTTC36190.16777473402442863No Hit
TAGGCCGCCTGAATCCAAAAACGGCACGGTGATGAGTCAAGAAGCAGGAGAAAG35450.16434413708665363No Hit
G35240.16337058930701479No Hit
TAGGCCGCTTGATCTGGAGGAAGAGAATGGAATACTAAGGTGAAACGAGCAAGC34410.15952275760653742No Hit
TAGGCCTGTAAGCTTTTCTTGGCTAAGGATGGCGTCAGACAAGGCGCGCCCTGT34310.15905916342575702No Hit
CGAGCTGCACAGCGGATGGCAAAGTGAAAGTGAACGGTGTTGGCG33320.154469581036031No Hit
CCCCTTTTCCCCCTCTTCCACCACAGAGCCTCATCACCCGAATTCTGCCGCCCT32340.14992635806438304No Hit
CGAAGTAGCAGTCACGAGTGGGATTTTGGCGCAGATGTGAGCGTTTCAC32190.1492309667932124No Hit
CGACCGCTTCCCCCCTCCCGTCGCTCGTCCAGCCTTCTTGGCATTCGCC31270.14496590033003268No Hit
CGAAGTACACGGTAGTCAAGGCTGTTCGTTAAATGTCTTTTTCAAGTAG31240.14482682207579858No Hit
CGATCGGAGTTGCAGATTAGGTCCTTGTTCGTCAGTTCCGTTATCTCGC30420.14102534979339926No Hit
TAGGCCTCGTGCGATTCGGTAAGGGAGGCGGGTTAGCGAGGAGTGGAATGTTGC29580.13713115867484385No Hit
TAGGCCTTTCTTACTACTTGTATACAGAAGATCGTGAAACGCCTCCAAATGCTG29340.13601853264097089No Hit
TAGGCCTCGTGTCTTTTAGTACAACCGATGCGACAGGAGTCCAGGAAGAGGTAG28560.13240249803088372No Hit
TAGGCCGCTGGCAAGGGGTTTGCTTAAATGACCTGGTGAGCTTTCTGTTTGTTT28370.13152166908740093No Hit
TAGGCCTTCTCTTGGCACGTGTTTTGGGAGTGACGATGTCGATGTGGAAGTAAA28260.13101171548854249No Hit
CGACCAGGTCAGCTCGGCTTGCCGCCACAGCACAACCTGGCTTGTAGCT27900.12934277643773306No Hit
AAATACCAAAGCAAAGATCGATTGATAGCACGATTGGTGACTGTAGGTGATGGG26400.12238886372602698No Hit
CGAGCAATGTGGTTCTACTTGGGCATGTGAAAGAGGCAAGTCCGTGTAG25540.11840195377131547No Hit
CGATGGCTCACTGGTGTAGAAGTTGTTGTCGTGAGGCAGCTAGATGCAT25480.11812379726284725No Hit
CGAAAGAAATCATGTACCTATGCAATTACAAACACCTTGGTCTCAAAGC25430.11789200017245703No Hit
CGATTTGAACGCACTTGACGTATTCGTTCAGGTAGCGATCCTAGATACG25330.11742840599167663No Hit
CGACTTGGATCCGAGACTGCATAAGTA25150.1165939364662719No Hit
CGAATTTGCTTACTAGACGTTCGAATCTCAGGCCAAATCCAAGTAGCAG25070.11622306112164758No Hit
CGAGTTAGCACGCCACGAGGCTGGCAAAGACGTTCCCGAGGACATTGTT24830.11511043508777462No Hit
TAGGCCGCTCGGTCAGCTGTGGTCAAGCTTAGAA23680.10977910200879995No Hit
CGAC23370.10834196004838069No Hit
CGACTGATGCAAGGACGGTACTTGCCGCTCACCTGTTCTTCAATTGATG22810.10574583263601041No Hit
TAGGCCTTGTTGGATGTTTTGTCGATGAGCACGGCGTTGCTAAAGAGAGTGTTT22610.1048186442744496No Hit
TAGGCCTCAGGAAGGGCTTTAGGCTTTGAGAACTGAAATGGCGAGGAAAGAAGG22450.10407689358520097No Hit
CGACTGGAACAACCTCGCCTCCCCAACCGTTCATCGTACCCCCCTTAGC22060.10226887628015738No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)